Gukoresha ultrafiltration mugutandukanya poroteyine no kwezwa

Ubuhanga bwa Ultrafiltration nubuhanga bushya kandi bunoze bwo gutandukanya tekinoroji.Ifite ibiranga inzira yoroshye, inyungu zubukungu nyinshi, nta gihinduka cyicyiciro, coefficente nini yo gutandukana, kuzigama ingufu, gukora neza, nta kwanduza kwakabiri, imikorere ikomeza kubushyuhe bwicyumba nibindi.Uyu munsi, umuyobozi Yang waturutse i Beijing yatubajije ibikoresho byacu bya ultrafiltration yo kweza poroteyine kandi avugana birambuye nikoranabuhanga ryacu.Noneho, umwanditsi w'itsinda rya Shandong Bona azamenyekanisha ikoreshwa rya ultrafiltration mugutandukanya poroteyine no kwezwa.

1. Kubwa poroteyine, gusindisha no kwibanda
Ibyingenzi byingenzi bya ultrafiltration mugusukura poroteyine ni uguhindura no kwibanda.Ultrafiltration uburyo bwo gusibanganya no kwibanda kurangwa nubunini bunini, igihe gito cyo gukora hamwe nuburyo bwiza bwo kugarura poroteyine.Uburyo gakondo bwo gutandukanya chromatografiya yo gukuraho ibintu bitandukanye muri poroteyine byasimbuwe nubuhanga bugezweho bwa ultrafiltration, bwabaye ikoranabuhanga nyamukuru ryo kuvanaho poroteyine, kunywa inzoga no kwibanda muri iki gihe.Mu myaka yashize, tekinoroji ya ultrafiltration yakoreshejwe cyane muguhindura no kugarura proteine ​​zifite intungamubiri nyinshi muri foromaje ya soya na soya.Lactose n'umunyu nibindi bice bigize poroteyine, hamwe nibikenewe nyabyo byo kurangiza neza desalting, de-alcool hamwe na proteine.Ikoreshwa rya tekinoroji ya ultrafiltration irashobora kandi kwibanda kuri serospecies immunoglobuline kugirango ihuze ibyifuzo bya proteine.

2. Kubice bya poroteyine
Gucamo poroteyine bivuga inzira yo gutandukanya buri kintu kigizwe na poroteyine ku gice ukurikije itandukaniro ryimiterere yumubiri nubumara (nkuburemere bwa molekile ugereranije, point ya isoelectric point, hydrophobicity, nibindi) bya buri kintu cya poroteyine mumazi yo kugaburira.Gel chromatografiya ni bumwe muburyo bukoreshwa mugucamo ibice bya macromolecules (cyane cyane proteyine).Ugereranije na chromatografiya gakondo, tekinoroji yo gutandukanya ultrafiltration ifite ibyiringiro byiza byo gukoreshwa mugucamo ibice no gukora inganda za poroteyine na enzymes bifite agaciro gakomeye mubukungu kubera igiciro cyacyo gito kandi cyoroshye.Amagi yera nibikoresho bihendutse kubona lysozyme na ovalbumin.Vuba aha, ultrafiltration ikoreshwa mugutandukanya ovalbumin na lysozyme n'umweru w'igi.

3. Gukuraho Endotoxine
Kurandura Endotoxine nimwe muburyo bukoreshwa muburyo bwa tekinoroji ya ultrafiltration mugusukura poroteyine.Igikorwa cyo gukora endotoxine kiragoye cyane.Muburyo bwo kubishyira mubikorwa, kubera ko poroteyine yimiti ikorwa na sisitemu yo kwerekana imiterere ya prokaryyotike byoroshye kuvanga na endotoxine ikorwa na bagiteri yamenetse, na endotoxine, izwi kandi nka pyrogen, ni ubwoko bwa lipopolysaccharide.Nyuma yo kwinjira mumubiri wumuntu, irashobora gutera umuriro, guhungabana kwa microcirculation, ihungabana rya endotoxic nibindi bimenyetso.Kugirango urinde ubuzima bwabantu, birakenewe gukoresha tekinoroji ya ultrafiltration byimazeyo kugirango ukureho endotoxine.

Nubwo tekinoroji ya ultrafiltration ikoreshwa cyane mugutandukanya no kweza poroteyine, nayo ifite aho igarukira.Niba uburemere bwa molekuline yibicuruzwa byombi bigomba gutandukana bitarenze inshuro 5, ntibishobora gutandukanywa na ultrafiltration.Niba uburemere bwa molekuline bwibicuruzwa buri munsi ya 3kD, ntibishobora kwibanda kuri ultrafiltration, kubera ko ultrafiltration ikorwa muburemere buke bwa molekuline ya 1000 NWML.

Hamwe niterambere rihoraho ryinganda za bioengineering, ibisabwa byashyizwe imbere kugirango bitandukane kandi byikoranabuhanga.Uburyo bwa gakondo bwo kwibanda kuri vacuum, gukuramo ibishishwa, dialyse, centrifugation, imvura no gukuraho pyrogene ntabwo byemewe kugirango bikemure umusaruro ukundi.Tekinoroji ya Ultrafiltration igomba gukoreshwa cyane kuko ibyiza byayo mu gutandukanya poroteyine.